DNA

Part:BBa_K4765132

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-08)


ribozyme connected: intimin-Nb2 + mScarlet contributed by Fudan iGEM 2023


Usage and Biology

This composite part is the version of BBa_K4765104 with mScarlet added. We use this part to observe the biofilm formation process.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(2) indicate the successful construction of intimin-Nb2 and intimin-Nb2 + mScarlet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3012
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305


[edit]
Categories
//collections/biofilm
Parameters
proteins